Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains

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Title
Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains
Author(s)
Byoung Chul Park; Hyang Sook Lim; Myung Hee Kim; Dae Sil Lee
Bibliographic Citation
Korean Biochemical Journal, vol. 21, no. 3, pp. 214-220
Publication Year
1988
Abstract
Total synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coll using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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