Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains

Abstract

Total synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coll using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed.