Production of ser125-interleukin-2 by site directed mutagenesis

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Title
Production of ser125-interleukin-2 by site directed mutagenesis
Author(s)
Seongman Kang; Ju Won Kwak; Jong Bum Kwon; Sung Wan Kim; In Young Lee; Sun Bok Lee; Hye-Young Yun; Kyung Soo Hahm; Moon Hi Han; Doe Sun Na
Bibliographic Citation
Korean Biochemical Journal, vol. 22, no. 3, pp. 306-311
Publication Year
1989
Abstract
Human Interleukin-2 (IL-2) posessing a serine in place of cystein at the amino acid sequence position 125 has been produced in E. coll by site directed mutagenesis. The activity of purified Ser125-IL-2 was more than 2.5 times higher than that of Cys125-IL-2 as determined by the growth promoting effect on IL-2 dependent cell line. The yield of production of Ser125-IL-2 was at least 1.5 times higher as compared to that of Cys125-IL-2.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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