Cloning and expression of human alpha1-antitrypsin cDNA in yeast Saccharomyces cerevisiae = 효모에서 인체 alpha1-antitrypsin cDNA의 클로닝 및 발현

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Title
Cloning and expression of human alpha1-antitrypsin cDNA in yeast Saccharomyces cerevisiae = 효모에서 인체 alpha1-antitrypsin cDNA의 클로닝 및 발현
Author(s)
Jinmi Kim; Kee Nyung Lee; Myeong Hee Yu
Bibliographic Citation
Korean Biochemical Journal, vol. 23, no. 2, pp. 263-268
Publication Year
1990
Abstract
Human a 1-antitrypsin cDNA was expressed in yeast Saccharomyces cerevisiae under the regulation of GAL10-CYC1 hybrid promoter. A suitable Gal+ host strain was constructed by tetrad analysis of a diploid from a cross of two Gal' strains, each affected in a different galactose utilizing gene. The resulting strain KY8 was transformed with the expression plasmid pGAT7 and the synthesis of a 1-antitrypsin was induced in galactose medium. Production of al-antitrypsin in recombinant yeast cells was confirmed by an elastase inhibitory assay and an immunoblotting method. The molecular weight of recombinant al-antitryp-sin synthesized in yeast was 44 kDa which was expected from the full length cDNA sequence of the crl-antitrypsin structural gene.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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