Cloning and expression of human lipocortin-1 cDNA in E. coli = 인간 리포코틴-1 유전자의 클로닝 및 대장균에서의 발현

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Title
Cloning and expression of human lipocortin-1 cDNA in E. coli = 인간 리포코틴-1 유전자의 클로닝 및 대장균에서의 발현
Author(s)
Kwahng Rae Huh; Soon Hee Park; Sung Mang Kang; In Sung Song; Hay Young Lee; Doe Sun Na
Bibliographic Citation
Korean Biochemical Journal, vol. 23, no. 4, pp. 459-464
Publication Year
1990
Abstract
A cDNA clone coding for lipocortin-1 was isolated from the cDNA library of human placental mRNA. Plasmid pHTl was constructed by inserting the coding sequence into plasmid pKK233-2, a universal expression vector containing trc promoter. Lipocortin-1 was expressed constitutively or by induction with IPTG in E. coli strain C600 or JM109 harboring pHTl. The activity of purified lipocortin-1 was demonstrated by the in vitro phospholipase A2 inhibitory activity.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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