Production of recombinant human interleukin-2 by Escherichia coli with computer controlled temperature induction

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Title
Production of recombinant human interleukin-2 by Escherichia coli with computer controlled temperature induction
Author(s)
Bong Hyun Chung; Dong Jin Seo; Young Hoon Park; Sun Bok Lee; Moon Hi Han
Bibliographic Citation
Biotechnology Techniques, vol. 5, no. 3, pp. 163-168
Publication Year
1991
Abstract
The amount of alkali added to the fermentation broth of a recombinant Escherichia coli strain for pH control was monitored on-line by an electronic balance interfaced to a computer. It was successfully correlated with the cell mass, and consequently the cell growth could be well estimated. Using this cell growth estimation technique, an automatic temperature induction was successfully carried out to produce human interleukin-2 in a high yield from E. coli harbouring a temperature controlled expression vector system.
Keyword
Escherichia ColiFermentationCell GrowthExpression VectorControl Temperature
ISSN
0951-208X
Publisher
Unknown
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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