Rapid and simple purification of human immunodeficiency virus 1 epitope gp41

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Title
Rapid and simple purification of human immunodeficiency virus 1 epitope gp41
Author(s)
Mi Jin Sohn; Seo Hee Cho; Won-Hee Jang; Young-Hae Chong; Sang Uk Nham; Young Ik Lee
Bibliographic Citation
Journal of Virological Methods, vol. 41, no. 1, pp. 93-100
Publication Year
1993
Abstract
In order to develop a reliable and inexpensive serodiagnostic method, part of the transmembrane glycoprotein gene of HIV-1, gp41', (HIV-env 548-646) was cloned into an expression vector, pCT10 with a sequence encoding a hydroxylamine cleavage site and with a part of Lac Z gene (Lac 2'': 834 base pairs) as a fusion partner. Overexpression of Lac Z''-gp41' was induced in E. coli and the gp41' fusion protein was purified to homogeneity by centrifugation, hydroxylamine cleavage and an ion-exchange chromatography. Western blot analysis and enzyme-linked immunosorbant assay (ELISA) using the purified gp41 fragment showed high sensitivity and specificity of gp41 as an antigen to detect anti HIV-1 antibodies in testing human sera. These results suggest that this simple and rapid purification method is reliable for obtaining a large quantity of purified gp41'.
Keyword
gp41human immunodeficiency virus-1purification
ISSN
0166-0934
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0166-0934(93)90165-N
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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