Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus

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Title
Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus
Author(s)
Byung Rae Jin; Chun Jeih Ryu; Sung Sup Park; Uk NangGung; Hyo Jeong Hong; Moon Hi Han
Bibliographic Citation
Molecular Immunology, vol. 30, no. 18, pp. 1647-1654
Publication Year
1993
Abstract
The cloning, expression and characterization of a murine-human chimeric antibody with specificity for the pre-S2 surface antigen (Ag) of hepatitis B virus (HBV) is described. The heavy and light chain variable region (VH and VL) genes encoding the murine monoclonal antibody (mAb) were isolated and combined with human γ 1 and κ constant region genes, respectively. The expression vectors containing the chimeric heavy and light chain genes were sequentially electroporated into murine Sp2/0 hybridoma cells and transfectomas secreting chimeric antibody were isolated. The chimeric antibody was purified and characterized by ELISA, Western analysis and competition immunoassay, demonstrating that the transfectoma functionally express and secrete murine-human chimeric antibody which retained the specificity and affinity of the parental murine mAb.
ISSN
0161-5890
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0161-5890(93)90438-H
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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