Determination of genome size and a preliminary physical map an extreme alkaliphile Micrococcus sp. Y-1 by pulsed-field gel electrophoresis
Cited 6 time in
- Title
- Determination of genome size and a preliminary physical map an extreme alkaliphile Micrococcus sp. Y-1 by pulsed-field gel electrophoresis
- Author(s)
- Jong Hoon Park; Jae-Chan Song; Myung Hee Kim; Dae Sil Lee; Chul Ho Kim
- Bibliographic Citation
- Microbiology-SGM, vol. 140, pp. 2247-2250
- Publication Year
- 1994
- Abstract
- Large restriction fragments of genomic DNA from Micrococcus sp. Y-1 were separated by pulsed-field gel electrophoresis (PFGE). Since Micrococcus sp. Y-1 has a G+C content of approximately 70%, restriction fragments were obtained by digesting chromosomal DNA with endonucleases which recognize A+T-rich sequences. Five enzymes, SspI, SpeI, XbaI, HpaI and EcoRI, were used for generation of distinctly separated fragments in the size range 100-500 kb. No site for DraI was detected. In contrast, sites for 8-base-recognizing enzymes, but not for NotI and SfiI, were frequent. The genome size of Micrococcus sp. Y-1 was determined from restriction fragments separated by PFGE, and was estimated to be approximately 4061 kb. Partial digestion experiments revealed the order of the six SspI fragments on the chromosome.
- Keyword
- alkaliphilegenome sizemicrococcus sp.
- ISSN
- 1350-0872
- Publisher
- Microbiology Soc
- DOI
- http://dx.doi.org/10.1099/13500872-140-9-2247
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.