Tomato plants transformed with cDNA of CMV I/sub17/N-Satellite RNA: differential resistance to CMV-K and CMV-Y

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Title
Tomato plants transformed with cDNA of CMV I/sub17/N-Satellite RNA: differential resistance to CMV-K and CMV-Y
Author(s)
Sang Jik Lee; Byung-Dong Kim; Kyung Hee Park
Bibliographic Citation
Molecules and Cells, vol. 4, no. 1, pp. 67-71
Publication Year
1994
Abstract
cDNA clone of cucumber mosaic virus (CMV) II7N-satellite RNA was introduced into tomato plants (Lycopersicon esculentum cv. Pink Forcer) by Agrobacterium tumefaciens Ti plasmid mediated transformation. From PCR and Northern blot analyses, it was confirmed that the cDNA of satellite RNA was integrated into the plant genome and stably expressed in the transgenic tomato plants. Transgenic plants producing CMV satellite RNA were challenged with CMV-K or CMV-Y. In the case of CMV-Y inoculated transgenic plants, there was almost no development of disease symptoms, and production of viral RNAs was greatly reduced when compared with the CMV-Y inoculated nontransformed control plants. On the other hand,development of disease symptoms was barely attenuated and only sUght reduction of viral RNAs was observed in the case of CMV-K inoculated transgenic plants when compared with the CMV-K inoculated nontransformed plants.
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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