Synthesis of an aspartame precursor using immobilized thermolysin in an organic solvent

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Title
Synthesis of an aspartame precursor using immobilized thermolysin in an organic solvent
Author(s)
Kyung Seop Ahn; In Young Lee; Ik Hwan Kim; Young Hoon Park; Sun Bok Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 4, no. 3, pp. 204-209
Publication Year
1994
Abstract
N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, a precursor of the synthetic sweetener, aspartame, was synthesized from N-(benzyloxycarbonyl)-L-aspartic acid and L-phenylalanine methyl ester with an immobilized thermolysin (EC 3.4.24.4) in the mixed organic solvent system of tert-amyl alcohol and ethyl acetate. A mixed solvent consisting of tert-amyl alcohol and ethyl acetate at a ratio of 33:67 (v/v) was found to be the most suitable with respect to synthetic rate and stability of the immobilized enzyme. The reaction continued to proceed quite successfully in a column reactor at 40 degrees C and at a space velocity of 3.6 h(-1) with a yield of 99%, using 40 mM Z-Asp and 200 mM PheOMe dissolved in the mixed solvent as the substrate.
Keyword
aspartame precursorenzymatic processorganic single phase
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1002/bit.260460617
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
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