Expression of human lactoferrin in milk of transgenic mice using bovine beta-casein/human lactoferrin cDNA fusion gene

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Title
Expression of human lactoferrin in milk of transgenic mice using bovine beta-casein/human lactoferrin cDNA fusion gene
Author(s)
Sun Jung Kim; Yong-Yeon Cho; Ko Woon Lee; Dae Yeul Yu; Chul Sang Lee; Yong Mahn Han; Kyung Kwang Lee
Bibliographic Citation
Molecules and Cells, vol. 4, no. 3, pp. 355-360
Publication Year
1994
Abstract
on II, and SV40 polyadenylation signal to control the expression of hLF cDNA. Twenty lines of transgenic mice were produced. Milk could be collected from four lines of transgenic mice. In two of them hLF was present in the milk at concentrations of 150?200 pg/ml. Mammary-specific hLF expression was not correlated to the transgene copy number. hLF RNA was only detected in the mammary gland of transgenic mice. The expressed RNA was correctly spliced at the exon/intron junctions. From these results, it may be concluded that the recombinant expression vector developed in this study is a good candidate to control high levels of foreign gene expression in the mammary gland of transgenic animals.
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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