Large scale purification of KRF-001 on the preparative HPLC = Preparative HPLC를 이용한 KRF-001의 대량분리정제

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Title
Large scale purification of KRF-001 on the preparative HPLC = Preparative HPLC를 이용한 KRF-001의 대량분리정제
Author(s)
Hang-Woo Lee; Moo-Kyung Kim; Tae Sook Jeong; Song Hae Bok
Bibliographic Citation
Korean Journal of Biotechnology and Bioengineering, vol. 9, no. 4, pp. 385-394
Publication Year
1994
Abstract
Large sc le purification to get antifungal antibiotic KRF-001 of 90% purity, was investigated using preparative HPLC. Crude KRF-001 was purified by XAD-7 adsorption chromatography, acid precipitation and mi:rofiltration. Microfiltration was the most effective isolation method of crude KRF-001. The purification methods using Cl8 chromatography was convenient compared with the conventional methods. Delta 1 AK C18 column and Bonda PAK CIS column were adapted large scale purification of KRF-001. Gradie it system of prep HPLC using Del切 PAK Cu column was more effective. With these conditions, final ecovery of KRF-001 yielded 77%.
ISSN
1225-7117
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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