Identification of 4-demethylsterols from suspension cultured cells of Marchantia polymorpha L. = Marchantia polymorpha( 우산이끼 ) 현탁배양 세포내의 4-demethylsterol 들의 동정

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Title
Identification of 4-demethylsterols from suspension cultured cells of Marchantia polymorpha L. = Marchantia polymorpha( 우산이끼 ) 현탁배양 세포내의 4-demethylsterol 들의 동정
Author(s)
K W Ko; K S Han; S J Nam; Sang Soo Kwak; J K Shim; S K Kim
Bibliographic Citation
Korean Journal of Botany, vol. 38, no. 3, pp. 219-225
Publication Year
1995
Abstract
Five kinds of 4-demethylsterol were isolated from suspension cultured cells of a liverworth, Marchantia polymorpha. Four 4-demethylsterols among them were analyzed by a capillary gas chromatography-mass spectrometry and 500 MHz 1H-NMR, and characterized to be avenasterol, 24-methylene-cholesterol, stigmasterol, and campesterol. And the fifth 4-demethylsterol was characterized to be sitosterol by a capillary gas chromatography-mass spectrometry. The concentration of the 4-demethylsterols in the cell decreased in order of avenasterol gt stigmasterol gt 24-methylene-cholesterol gt campesterol gt sitosterol. When carbon skeleton and the oxidation state was compared with those of brassinosteroids, these 4-demethylsterols may be potent biosynthetic precursors of brassinosteroids in the cells.
Keyword
Marchantia polymorphasuspension cultured cell4-demethylsterolsstructure determinationprecusors of brassinosteroids
ISSN
I000-0012
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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