자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정

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Title
자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정
Author(s)
Young Keun Mo; Young Keun Shin; Dong Jin park; Chang Jin Kim; Chong Kil Lee; Seong Sun Han
Bibliographic Citation
Yakhak Hoeji, vol. 39, no. 6, pp. 585-592
Publication Year
1995
Abstract
Soil microorganisms producing immunomodulators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to 180 J/m2 of UVB radiation resulted in approximately 90% impairment of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supernatants of 150 actinomycete strains were examined for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell function. The soil isolate that showed the most effective restorative activity, G40025, was selected and further characterized. Addition of 10mcl of the culture supernatant of G4002 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodulator produced by G40025 appeared to be stabled at 100oC for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G4002, belongs to the genus Streptomyces.
Keyword
Ultraviolet BLangerhans cellsimmunomodulatorsTaxonomyStreptomyces sp.
ISSN
0513-4242
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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