Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus

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Title
Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus
Author(s)
Byung Sik Shin; Seung Hwan Park; Soo Keun Choi; Bon Tag Koo; Sung-Taik Lee; Jeong Il Kim
Bibliographic Citation
Applied and Environmental Microbiology, vol. 61, no. 6, pp. 2402-2407
Publication Year
1995
Abstract
DNA dot blot hybridizations with a cryV-specific probe and a cryI- specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific probe hybridized to 12 of the B. thuringiensis strains examined. Most of the cryV-positive strains also hybridized to the cryI-specific probe, indicating that the cryV genes are closely related to cryI genes. Two cryV-type genes, cryV1 and cryV465 were cloned from B. thuringiensis subsp. kurstaki HD-1 and B. thuringiensis subsp. entomocidus BP465, respectively, and their nucleotide sequences were determined. The CryV1 protein was toxic to Plutella xylostella and Bombyx mori, whereas the CryV465 protein was toxic only to Plutella xylostella.
Keyword
bacillus thuringiensisgene locationbacillus thuringiensisbacterial proteinsgene expression
ISSN
0099-2240
Publisher
Amer Soc Microb
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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