Sequences responsible for transcription termination of the mouse gastrin gene

Cited 0 time in scopus
Metadata Downloads
Title
Sequences responsible for transcription termination of the mouse gastrin gene
Author(s)
Moon Jong Noh; Sun Jung Kim; Yong Koo Kang; Ook Joon Yoo
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 35, no. 6, pp. 1205-1213
Publication Year
1995
Abstract
To identify the transcription termination elements in the mouse gastrin gene, we examined RNA transcripts after stable transfection of gastrin expression plasmids into the NIH3T3 cell line. The GT-repeat region at the 3'-flanking sequence of the mouse gastrin gene acted as a transcription terminator. When the GT-repeat unit was deleted from its site, the effect of termination disappeared. Further experiment, using serial deletion mutants, revealed that the 56-38 nucleotide upstream region from the GT-repeat unit also participated in transcription termination. We propose that the upstream region of the GT-repeat unit might be recognized as a pause site by the RNA polymerase II, and an abnormal DNA structure, derived from the GT-repeat unit, might function as a blockage of polymerase processivity.
ISSN
1039-9712
Publisher
Wiley
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.