Regulation of poly­β­hydroxybutyrate biosynthesis by nicotinamide nucleotide in Alcaligenes eutrophus

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dc.contributor.authorIn Young Lee-
dc.contributor.authorMi Kyung Kim-
dc.contributor.authorHo Nam Chang-
dc.contributor.authorYoung Hoon Park-
dc.date.accessioned2017-04-19T08:45:04Z-
dc.date.available2017-04-19T08:45:04Z-
dc.date.issued1995-
dc.identifier.issn0378-1097-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3629-
dc.description.abstractPoly-β-hydroxybutyrate biosynthesis was studied in Alcaligenes eutrophus under various nutrient-limiting conditions. When the cells were cultivated in nitrogen-limited media, both the levels of NAD(P)H and the ratios of NAD(P)H/NAD(P) were higher than those under nitrogen-sufficient conditions. The specific poly-β-hydroxybutyrate production rate was found to increase with the values of both NADH/NAD and NADPH/NADP, indicating that poly-β-hydroxybutyrate synthesis is directly regulated by the ratios of nicotinamide nucleotides. The effects of nicotinamide nucleotides on poly-β-hydroxybutyrate biosynthesis was investigated with regard to enzyme kinetics. Citrate synthase activity was significantly inhibited by NADH and NADPH, indicating that poly-β-hydroxybutyrate accumulation could be enhanced by facilitating the metabolic flux of acetyl-CoA to poly-β-hydroxybutyrate synthetic pathway. It was also found that cellular NADPH was a limiting substrate for NADPH-linked reductase, controlling the overall biosynthetic activity of poly-β-hydroxybutyrate in this strain.-
dc.publisherOxford Univ Press-
dc.titleRegulation of poly­β­hydroxybutyrate biosynthesis by nicotinamide nucleotide in Alcaligenes eutrophus-
dc.title.alternativeRegulation of poly­β­hydroxybutyrate biosynthesis by nicotinamide nucleotide in Alcaligenes eutrophus-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number0-
dc.citation.endPage39-
dc.citation.startPage35-
dc.citation.volume131-
dc.contributor.affiliatedAuthorIn Young Lee-
dc.contributor.affiliatedAuthorMi Kyung Kim-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeName이인영-
dc.contributor.alternativeName김미경-
dc.contributor.alternativeName장호남-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 131, pp. 35-39-
dc.identifier.doi10.1111/j.1574-6968.1995.tb07333.x-
dc.subject.keywordalcaligenes eutrophus-
dc.subject.keywordnicotinamide nucleotide-
dc.subject.keywordpoly-β-hydroxybutyrate biosynthesis-
dc.subject.keywordregulation-
dc.subject.localAlcaligenes eutrophus-
dc.subject.localalcaligenes eutrophus-
dc.subject.localnicotinamide nucleotide-
dc.subject.localpoly-β-hydroxybutyrate biosynthesis-
dc.subject.localregulation-
dc.description.journalClassY-
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