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- Title
- Regulation of polyβhydroxybutyrate biosynthesis by nicotinamide nucleotide in Alcaligenes eutrophus
- Author(s)
- In Young Lee; Mi Kyung Kim; Ho Nam Chang; Young Hoon Park
- Bibliographic Citation
- FEMS Microbiology Letters, vol. 131, pp. 35-39
- Publication Year
- 1995
- Abstract
- Poly-β-hydroxybutyrate biosynthesis was studied in Alcaligenes eutrophus under various nutrient-limiting conditions. When the cells were cultivated in nitrogen-limited media, both the levels of NAD(P)H and the ratios of NAD(P)H/NAD(P) were higher than those under nitrogen-sufficient conditions. The specific poly-β-hydroxybutyrate production rate was found to increase with the values of both NADH/NAD and NADPH/NADP, indicating that poly-β-hydroxybutyrate synthesis is directly regulated by the ratios of nicotinamide nucleotides. The effects of nicotinamide nucleotides on poly-β-hydroxybutyrate biosynthesis was investigated with regard to enzyme kinetics. Citrate synthase activity was significantly inhibited by NADH and NADPH, indicating that poly-β-hydroxybutyrate accumulation could be enhanced by facilitating the metabolic flux of acetyl-CoA to poly-β-hydroxybutyrate synthetic pathway. It was also found that cellular NADPH was a limiting substrate for NADPH-linked reductase, controlling the overall biosynthetic activity of poly-β-hydroxybutyrate in this strain.
- Keyword
- alcaligenes eutrophusnicotinamide nucleotidepoly-β-hydroxybutyrate biosynthesisregulation
- ISSN
- 0378-1097
- Publisher
- Oxford Univ Press
- DOI
- http://dx.doi.org/10.1111/j.1574-6968.1995.tb07333.x
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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