Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha
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Title
Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha
Author(s)
Chul Ho Kim; Jung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 4, pp. 417-422
Publication Year
1996
Abstract
A heterologous gene from bloodsucking leech for anticoagulant, hirudin, has been expressed in the methylotrophic yeast Hansenula polymorpha. The addition of 1% (v/v) soybean oil to the medium as a stabilizer enhanced the expression of the hirudin gene in H. polymorpha with MOX promoter. The production of hirudin in the medium with soybean oil was 320 mg/L in a 5 L fermenter.