Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha

Cited 6 time in scopus
Metadata Downloads
Title
Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha
Author(s)
Chul Ho KimJung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 4, pp. 417-422
Publication Year
1996
Abstract
A heterologous gene from bloodsucking leech for anticoagulant, hirudin, has been expressed in the methylotrophic yeast Hansenula polymorpha. The addition of 1% (v/v) soybean oil to the medium as a stabilizer enhanced the expression of the hirudin gene in H. polymorpha with MOX promoter. The production of hirudin in the medium with soybean oil was 320 mg/L in a 5 L fermenter.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00143463
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.