Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase

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Title
Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase
Author(s)
Seung Goo Lee; Seung Pyo Hong; Moon Hee Sung
Bibliographic Citation
Enzyme and Microbial Technology, vol. 19, pp. 374-377
Publication Year
1996
Abstract
This study explores an enzymatic method for removing phenol from the wastewater created during the manufacture of phenolic resin. The enzyme used was a thermostable β-tyrosinase catalyzing the synthesis of L-tyrosine from phenol, pyruvate, and ammonia. As the reaction proceeds, L-tyrosine precipitates as insoluble aggregates because L-tyrosine is barely soluble in water. The enzymatic removal of phenol was effective at pH values ranging from 6.5-9.0 and temperatures below 70°C. The optimal concentration of each substrate was determined as 60 mM phenol, 0.1 M pyruvate, and 0.4 M ammonia. When the enzyme was used in an intact cell or acetone-dried cell state instead of the cell-free extract, the optimal concentration of phenol was increased up to 120 mM. By treating wastewater containing 100 mM phenol with acetone-dried cells at 37°C, we could reduce the concentration of phenol to 8 mM within 24h.
Keyword
enzymatic removal of phenolthermostable β-tyrosinasewastewater treatment
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0141-0229(96)00001-4
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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