Enzymatic conversion of glutaryl 7-aminocephalosporanic acid to 7-aminocephalosporanic acid with an immobilized glutaryl 7-aminocephalosporanic acid acylase

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Title
Enzymatic conversion of glutaryl 7-aminocephalosporanic acid to 7-aminocephalosporanic acid with an immobilized glutaryl 7-aminocephalosporanic acid acylase
Author(s)
Han Jae Shin; Seung Goo Lee; Wang-Sik Lee; Ki Hong Yoon
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 6, no. 5, pp. 336-339
Publication Year
1996
Abstract
Ghitaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30% of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be 30°C and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 ml of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60 mM GL 7-ACA within 8 h, resulting in a conversion yield of 83%.
Keyword
7-aminocephalosporanic acidglutaryl 7-aminocephalosporanic acid acylaseimmobilizationpseudomonas sp.
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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