Production of varicella-zoster virus using human lung fibroblast cells as host cells = 인체 폐섬유아세포 배양에 의한 수두바이러스의 생산

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Title
Production of varicella-zoster virus using human lung fibroblast cells as host cells = 인체 폐섬유아세포 배양에 의한 수두바이러스의 생산
Author(s)
Won-Bae Kim; Jung-Keug Park; Weon Sang Choi; Soo-Ok Kim; Ong-Ju Chung; Hyun-Su Kim; Ik Hwan Kim
Bibliographic Citation
Korean Journal of Biotechnology and Bioengineering, vol. 11, no. 2, pp. 254-261
Publication Year
1996
Abstract
Attenuated varicella-zoster virus (VZV) was propagated in human lung fibroblast (HLF) cells. Among media tested in this work,DMEM was the best medium for the growth of HLF cells. Because HLF was a normal finite cell line, cell growth rate was dependent on the age of HLF cells. When the population doubling level (PDL) was higher than 46, apoptosis of HLF cells started and cell growth rate decreased. The optimum temperature for the cell growth and virus propagation in the T-flask culture was 37°C. In a microcarrier culture system in which Cytodex-3 was used for the VZV propagation in spinner vessels, the yield of plaque forming cells was lower than that in the T-flask culture. The relatively high shear environment near microcarriers was thought to cause the low yield of VZV in the microcarrier culture system.
ISSN
1225-7117
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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