Inhibition of histamine synthesis by glycyrrhetinic acid in mast cells cocultured with Swiss 3T3 fibroblasts

Cited 17 time in scopus
Metadata Downloads
Title
Inhibition of histamine synthesis by glycyrrhetinic acid in mast cells cocultured with Swiss 3T3 fibroblasts
Author(s)
Young-Mi Lee; S Hirota; T Jippo-Kanemoto; Hyung-Ryong Kim; Tae-Yong Shin; Young Il Yeom; Kyung Kwang Lee; Y Kitamura; S Nomura; Hyung-Min Kim
Bibliographic Citation
International Archives of Allergy and Immunology, vol. 110, pp. 272-277
Publication Year
1996
Abstract
The effect of glycyrrhetinic acid (18-O-β-glycyrrhetinic acid, GA) on histamine metabolism was investigated in cultured mast cells (CMCs) cocultured with Swiss 3T3 fibroblasts. GA strongly inhibited histamine synthesis in the cocultured CMCs. Since 50 μM GA inhibited about 80% of histidine decarboxylase (HDC) activity, the inhibitory activity of GA for histamine synthesis was considered to be derived from the inhibition of HDC activity. The number of berberine-sulfate-positive cells also decreased in the presence of GA, which indicated that maturation of CMCs was inhibited by GA. Futhermore, we examined the effect of GA on the mRNA expression of novel protein kinase C δ (nPKCδ), a major isoform of CMCs, by Northern blot analysis. The expression of nPKCδ mRNA in the presence of GA was significantly lower than in the absence of GA. These results suggest the possibility that the inhibition of histamine synthesis by GA is regulated by nPKCδ.
Keyword
Glycyrrhetinic acidHistidine decarboxylaseProtein kinase CStem cell factor
ISSN
1018-2438
Publisher
Karger
DOI
http://dx.doi.org/10.1159/000237298
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.