High-level expression of human alphaS1-casein in Escherichia coli

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Title
High-level expression of human alphaS1-casein in Escherichia coli
Author(s)
Y K Kim; Bong Hyun Chung; S Yoon; Kyung Kwang Lee; B Lonnerdal; Dae Yeul Yu
Bibliographic Citation
Biotechnology Techniques, vol. 11, no. 9, pp. 675-678
Publication Year
1997
Abstract
Human αs1-casein was expressed efficiently in Escherichia coli. The overproduced recombinant human a αs1-casein was about 25% of the total cell protein. Two different vectors were constructed to express Met-αs1-casein and Met-αs1-casein with a His-affinity tag at the C-terminus. Recombinant Met-s1-casein with a His-affinity tag was purified to homogeneity using Ni-nitrilotriacetic acid resin. N-terminal sequence of the first 10 amino acid residues of this purified protein was identical to that of mature human αs1-casein with an extra methionine residue at the N-terminus.
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1018463411263
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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