Construction of a Clostridium acetobutylicum - Escherichia coli shuttle vector
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- Title
- Construction of a Clostridium acetobutylicum - Escherichia coli shuttle vector
- Author(s)
- Ki Hong Yoon; Jung Kee Lee; Byung Hong Kim
- Bibliographic Citation
- Biotechnology Letters, vol. 13, no. 1, pp. 1-6
- Publication Year
- 1991
- Abstract
- A 4.1-kb cryptic plasmid, designated pCA134, has been isolated fromClostridium species. In order to develop a vector suitable for transforming saccharolytic clostridia three hybrid plasmids were constructed by inserting pCA134 into pHV32 withEcoRI, orBglII andBamHI. The newly constructed plasmids were propagated inEscherichiacoli and were used to transformBacillussubtilis andClostridiumacetobutylicum. One of them, pCAB32 (10.1 kb), which contains chloramphenicol acetyltransferase gene and an origin of replication derived from pCA134 was introduced intoB.subtilis andC.acetobutylicum as well asE.coli.
- Keyword
- articleclostridium acetobutylicumescherichia colinonhumanrecombinant plasmidClostridium acetobutylicumEscherichia coli
- ISSN
- 0141-5492
- Publisher
- Springer
- Full Text Link
- http://dx.doi.org/10.1007/BF01033507
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
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