Construction of a Clostridium acetobutylicum - Escherichia coli shuttle vector

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Title
Construction of a Clostridium acetobutylicum - Escherichia coli shuttle vector
Author(s)
Ki Hong Yoon; Jung Kee Lee; Byung Hong Kim
Bibliographic Citation
Biotechnology Letters, vol. 13, no. 1, pp. 1-6
Publication Year
1991
Abstract
A 4.1-kb cryptic plasmid, designated pCA134, has been isolated fromClostridium species. In order to develop a vector suitable for transforming saccharolytic clostridia three hybrid plasmids were constructed by inserting pCA134 into pHV32 withEcoRI, orBglII andBamHI. The newly constructed plasmids were propagated inEscherichiacoli and were used to transformBacillussubtilis andClostridiumacetobutylicum. One of them, pCAB32 (10.1 kb), which contains chloramphenicol acetyltransferase gene and an origin of replication derived from pCA134 was introduced intoB.subtilis andC.acetobutylicum as well asE.coli.
Keyword
articleclostridium acetobutylicumescherichia colinonhumanrecombinant plasmidClostridium acetobutylicumEscherichia coli
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF01033507
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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