Flow cytometric analysis of antibody producing cells using double immunofluorescent staining

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Title
Flow cytometric analysis of antibody producing cells using double immunofluorescent staining
Author(s)
Gyun-Min Lee; Jae Hoon Kim; Hee Gu Lee
Bibliographic Citation
Biotechnology Techniques, vol. 10, no. 8, pp. 615-620
Publication Year
1996
Abstract
Double fluorescent labeling, with fluorescein isothiocyanate (FITC)- labeled F(ab')2 specific for the heavy chain and R-phycoerythrin (R-PE)- labeled F(ab')2 specific for the light chain, was demonstrated as a convenient means for the accurate evaluation of a heterogeneous non- antibody-producing population. Furthermore, it could be used for monitoring the changes in each immunoglobulin (Ig) chain content of the cells during the batch culture, which will facilitate the study on antibody synthesis, assembly and secretion.
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00157372
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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