Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae

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Title
Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae
Author(s)
Chi-Min Choi; Myoung-Dong Kim; Sang Ki Rhee; Jin-Ho Seo
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 10, pp. 1129-1132
Publication Year
1996
Abstract
The enriched medium based on yeast nitrogen base(YNB)increased hirudin synthesis and secretion in rccombinant Saccharomyces cerevisiae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudin/1 but supplementation of yeast extract increased the final hirudin concentration to 46lmg hirudin/1. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous culuture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00128579
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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