EcoRI variant N199H has enhanced specific activity

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Title
EcoRI variant N199H has enhanced specific activity
Author(s)
Jae Jong Kim; Kyung Tai Min; Myung Hee Kim; S J Augh; Byung-Dong Kim; Dae Sil Lee
Bibliographic Citation
Gene, vol. 171, pp. 129-130
Publication Year
1996
Abstract
The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (wild type; wt) > N199L > N199V > N199S > N199R > N199D. In particular, EcoRI variant N199H showed about a twofold higher specific activity than that of the wt enzyme.
Keyword
Site-directed mutagenesis
ISSN
0378-1119
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0378-1119(96)00003-0
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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