Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis

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Title
Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis
Author(s)
Mi Young Han; Mi Young Son; Seung-Hwan Lee; Jin Koo Kim; Jae Suk Huh; Jeong Hyun Kim; In Seong Choe; Tai Wha Chung; Yong Kyung Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 41, no. 4, pp. 657-663
Publication Year
1997
Abstract
A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1011 bp encoding isopropylmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.
Keyword
BCGIsopropylmalate dehydrogenase geneleuBM. tuberculosis
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549700201701
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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