Cloning of two chitin synthase gene fragments from Penicillium diversum = Penicillium diversum으로부터 두 chitin synthase 유전자 절편의 분리

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Title
Cloning of two chitin synthase gene fragments from Penicillium diversum = Penicillium diversum으로부터 두 chitin synthase 유전자 절편의 분리
Author(s)
Seong-Pil Cho; Sang-Keun Lee; Dong-Hun Lee; Kyung Sook Bae; Hee-Moon Park; Pil-Jae Maeng
Bibliographic Citation
Korean Journal of Mycology, vol. 25, no. 3, pp. 167-175
Publication Year
1997
Abstract
The PCR fragments of two distinct chitin synthase genes, PdCHSl and PdCHS2, were cloned from Penicillium diversum KCTC 6786. The nucleotide sequences of PdCHSl and PdCHS2 contained uninterrupted open reading frames (ORFs) of 570 bp excluding the primer sequence. The similarity analysis of the deduced amino acid sequences using BLASTP indicated that the possible evolutionary relationship between P. diversum and ascomycetous fungi. Multialignment of the deduced amino acid sequences of PdCHSs using CLASTAL W revealed that the PdCHSs fell into two different classes: PdCHSl into Class I and PdCHS2 into Class II of chitin synthase defined by Bowen et al. (1992). By Southern blot analysis, it was shown that each of the two genes is present as a single copy in the genome of P. diversum KCTC 6786.
Keyword
Chitin synthase genesPCR cloningPenicillium diversum
ISSN
0253-651X
Publisher
Korea Soc-Assoc-Inst
Type
Article
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1. Journal Articles > Journal Articles
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