Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae

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Title
Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae
Author(s)
Hyun Ah Kang; Moon Soo Jung; Won Kyoung Hong; Jung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 8, no. 1, pp. 42-48
Publication Year
1998
Abstract
In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GAL10, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.
Keyword
Saccharomyces cerevisiaehuman serum albuminsecretionprotein expressionprotein secretion
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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