Secretory expression of human α₁-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer

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Title
Secretory expression of human α₁-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer
Author(s)
Bong Hyun Chung; Su Jeong Kim; Hyun Ah Kang; Myeong Hee Yu
Bibliographic Citation
Biotechnology Letters, vol. 20, no. 3, pp. 307-311
Publication Year
1998
Abstract
Human α1-antitrypsin (α1-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of α1-AT, though the secretion efficiency was decreased. The final titre of α1-AT secreted and the secretion efficiency were 17.9 μg/ml and 24.8%, respectively, with 0.5 g galactose/l.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005350507744
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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