재조합 preS2 peptide에 대한 단일 클론항체 생산과 그를 이용한 면역 친화성 column 제조에 관한 연구

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Title
재조합 preS2 peptide에 대한 단일 클론항체 생산과 그를 이용한 면역 친화성 column 제조에 관한 연구
Author(s)
Mi Young Han; Sang Hoon Choi; In Seong Choe; Myung Ja Choi; Tai Wha Chung
Bibliographic Citation
Korean Biochemical Journal, vol. 22, no. 3, pp. 332-338
Publication Year
1989
Abstract
Three monoclonal antibodies specific to the recombinant preS₂ peptide were prepared using preS₂-β-galactosidase as an immunogen. All three were classified as immunoglobulin M. One of the three monoclonal antibodies (040-01) was precipitated by 6% PEG and used for the preparation of immunoadsorbent column. This immunoaffinity column was utilized to purify recombinant preS₂ peptide which was solubilized and extracted from sonicated E. coli cells. For the storage of this specific antibody, 0.1 M glycine-HCl buffer (pH 2.5) gave the best result. Other buffers tested in this study caused a significant decrease in antibody reactivity. The binding capacity of an immunoadsorbent column for preS₂ peptide was determined using preS₂-ABEI-H conjugate.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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