Cloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli

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Title
Cloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli
Author(s)
Kyung Hwa Jung; Kang Moon Lee; Hoon Kim; Ki Hong Yoon; Seung Hwan Park; Moo Young Pack
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 44, no. 2, pp. 283-292
Publication Year
1998
Abstract
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was catagorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes a xylanase having the molecular weight of 105 kilodaltons. A number of smaller truncated proteins with activities towards 4-methylumbelliferyl-β-D-cellobioside and xylan were also produced. The enzyme hydrolyzed xylan to xylo-oligosaccharide, indicating typical activity of endo-β-1,4-xylanase. This endoxylanase hydrolyzed carboxymethylcellulose without notable reduction of the viscosity as an exo-β-1,4-glucanase, even though the enzyme exhibited very low levels of activity against other soluble and insoluble cellulosic substrates.
Keyword
Clostridium thermocellumxylanasecloningxynXexoglucanase
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549800201302
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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