Characterization of monoclonal antibodies produced by hybridoma cell lines prepared against hepatitis B virus X protein

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Title
Characterization of monoclonal antibodies produced by hybridoma cell lines prepared against hepatitis B virus X protein
Author(s)
Mi Kyung Chung; Hee Gu Lee; Eun Suk Oh; Sung Shik Min; Hae Kyung Lee; Tae Gyu Lee; Jong Soon Lim; Chang Min Kim; Sue Nie Park
Bibliographic Citation
Animal Cell Technology & Applied Aspects, vol. 9, pp. 379-384
Publication Year
1998
Abstract
The X gene of HBV encodes HBx protein which is required for virus replication and has been implicated in virus mediated liver oncogenesis. However, the molecular mechanism of HBx activity and its possible influence on cell proliferation have to be solved further in detail. To understand more about the immune responses against HBV and to make tools useful for preparing diagnostic and therapeutic agents, we have produced monoclonal antibodies against HBx protein. Eight independent hybridoma cell lines including X-01A were isolated for this purified HBx protein. The isotype of the antibody produced from X-01A hybridoma cell line was IgGl. Some HBs-positive sera tested contained antibodies that specifically recognized the HBx protein. We conclude that the HBx protein is antigenic in some patients.
Keyword
Molecular Weight Standard MarkerGlutathione Agarose BeadKorea Cancer Center HospitalAcute Hepatitis PatientControl Clone
ISSN
I000-0064
DOI
http://dx.doi.org/10.1007/978-94-011-5161-0_65
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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