Cited 0 time in
- Title
- Rapid and simple purification of biologically active human hepatitis B virus transactivator-X proteins
- Author(s)
- Ha Ryoung Poo; Sun Ok Kim; Mi Jin Sohn; Sook Lee; Young Ik Lee
- Bibliographic Citation
- Journal of Microbiology, vol. 36, no. 1, pp. 55-58
- Publication Year
- 1998
- Abstract
- The human hepatitis B virus-X (HBV-X) was cloned into an expression vector, pET3d, containing a T7 promoter and direct expression was induced in Escherichia coli. The expressed HBV-X protein was purified to homogeneity by centrifugation, ion-exchange chromatography and gel filtration. After gel filtration, renaturation of HBV-X were performed using dialysis against serially diluted urea buffer. The biological activity of refolded HBV-X protein was confirmed by enhancement of protein/DNA complexes using gel-shift analysis.
- Keyword
- expressionpurificationHepatitis B virus-X protein
- ISSN
- 1225-8873
- Publisher
- Microbiological Society Korea
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.