Identification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe

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Title
Identification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe
Author(s)
Kwang Lae Hoe; Kyung Sook ChungMi Sun Won; O J Yoo; Hyang Sook Yoo
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 44, no. 5, pp. 897-906
Publication Year
1998
Abstract
By genomic Southern hybridization using degenerate oligo probes corresponding to the conserved zinc finger of the GATA family, four and three new GATA-factor genes were detected in Saccharomyces cereviaiae and Schizosaccharomyces and pombe respectively. Through degenerate PCR and Southern hybridization using an internal probe between the PCR primers, a new GATA-factor gene of S. pombe, gaf1, was identified. The DNA sequence analysis revealed that the partial gafl gene contains the strictly conserved zinc finger which is the hallmark of the GATA family. As judged form the codon usage of leucine in the zinc finger probe, the zinc fingers of S. pombe are more similar to those of higher fungi than those of S. cerevisiae, and this means that S. pombe is phylogenically close to higher organisms than S. cerevisiae.
Keyword
GATA factorzinc fingerS. pombedegenerate PCR
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549800201952
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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