Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies
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- Title
- Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies
- Author(s)
- Chul Ho Kim; H W Seo; H Y Kim; Jung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee
- Bibliographic Citation
- Journal of Industrial Microbiology & Biotechnology, vol. 21, pp. 1-5
- Publication Year
- 1998
- Abstract
- Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined.
- Keyword
- hirudinHansenula polymorphamethylotrophic yeastmethanol oxidaseautonomously replicating sequence
- ISSN
- 0169-4146
- Publisher
- Springer
- Full Text Link
- http://dx.doi.org/10.1038/sj.jim.2900545
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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