Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies

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Title
Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies
Author(s)
Chul Ho Kim; H W Seo; H Y Kim; Jung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 21, pp. 1-5
Publication Year
1998
Abstract
Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined.
Keyword
hirudinHansenula polymorphamethylotrophic yeastmethanol oxidaseautonomously replicating sequence
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.2900545
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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