Rapid method for the determination of lipid from the green alga Botryococcus braunii

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Title
Rapid method for the determination of lipid from the green alga Botryococcus braunii
Author(s)
Seog June Lee; Byung Dae Yoon; Hee-Mock OH
Bibliographic Citation
Biotechnology Techniques, vol. 12, no. 7, pp. 553-556
Publication Year
1998
Abstract
Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations.
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1008811716448
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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