Molecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator
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- Molecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator
- Kwang Lae Hoe; Mi Sun Won; Kyung Sook Chung; Seung Kiel Park; Dong Uk Kim; Young Joo Jang; Ook Joon Yoo; Hyang Sook Yoo
- Bibliographic Citation
- Gene, vol. 215, no. 2, pp. 319-328
- Publication Year
- As a first step to elucidate the functions of Schizosaccharomyces pombe (S. pombe) GATA factors, we have isolated the gaf1+ gene (GATA-factor like gene) in S. pombe. The predicted amino acid (aa) sequence of Gaf1 reveals a single zinc finger domain typical of fungal GATA factors, and the zinc finger exhibits 60% aa identity to that of human GATA-1. The open reading frame of Gaf1 predicts a protein of M(r) 32 kDa consisting of 290 intronless amino acids. Disruption of this gene has no effect on cell viability and growth rate. The GST-Gaf1 fusion protein binds specifically to GATA motifs of its own promoter as well as DAL7 UAS, a canonical GATA motif of Saccharomyces cerevisiae (S. cerevisiae) The specific DNA-binding activity resides within the N-terminal half of Gaf1 (Gaf1N; aa 1-120) containing the zinc finger, whereas the C-terminal half (Gaf1C; aa 121-290) contains transactivation sequences that induce the expression of the lacZ reporter when fused to the GAL4 DNA binding domain. These results demonstrate that Gaf1 may function as a transcriptional activator consisting of DNA-binding and transactivation domains.
- Zinc fingerGATA-bindingTranscription factorDegenerate PCRS. pombe
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- Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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