Cloning and expression of the β-galactosidase gene from Bifidobacterium longum KCTC 3215 in Escherichia coli = Bifidobacterium longum KCTC 3215의 β-Galactosidase 유전자의 cloning 및 대장균에의 발현

Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.