Cloning and expression of the β-galactosidase gene from Bifidobacterium longum KCTC 3215 in Escherichia coli = Bifidobacterium longum KCTC 3215의 β-Galactosidase 유전자의 cloning 및 대장균에의 발현
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- Title
- Cloning and expression of the β-galactosidase gene from Bifidobacterium longum KCTC 3215 in Escherichia coli = Bifidobacterium longum KCTC 3215의 β-Galactosidase 유전자의 cloning 및 대장균에의 발현
- Author(s)
- Hae Ki Min; Kook Hee Kang; Moon Hee Sung
- Bibliographic Citation
- 성균관대학교논문집-과학기술편, vol. 43, no. 2, pp. 795-803
- Publication Year
- 1993
- Abstract
- A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
- ISSN
- I000-0108
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
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