Cecropin A-magainin 2 hybrid peptides having potent antimicrobial activity with low hemolytic effect

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Title
Cecropin A-magainin 2 hybrid peptides having potent antimicrobial activity with low hemolytic effect
Author(s)
Song Yub Shin; Joo Hyun Kang; Myung Kyu Lee; Sun Young Kim; Yang Mee Kim; Kyung Soo Hahm
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 44, no. 6, pp. 1119-1126
Publication Year
1998
Abstract
In order to obtain peptides having improved antimicrobial activity with low hemolytic effect, a hybrid peptide (CA-MA) composed from cecropin A(1-8) and magainin 2(1-12), and its analogues with amino acid substitutions were designed and synthesized. The antimicrobial activities against bacterial cells and hemolytic activities against human red blood cells were analyzed for each peptide. Secondary structures of the peptides in aqueous solution, 50% trifluoroethanol, and sodium dodecylsulfate micelles were estimated using circular dichroism spectroscopy. The increase in hydrophobicity or α-helicity of the peptides correlated with an increase in hemolytic activity rather than antimicrobial activity. The substitution of Leu for Ser at position 16 in CA-MA resulted in a remarkable increase in antimicrobial activity without a significant change in hemolytic activity. Furthermore, the increase in antimicrobial activity of the peptides was not always accompanied by the increase in hemolytic activity.
Keyword
hydrophobicityantimicrobial activityCA-MA hybrid peptidehelicityhemolytic activity
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549800202192
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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