Molecular cloning and expression of human galβ1,3galNAc α2,3-Sialyltransferase(hST3Gal II)

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Title
Molecular cloning and expression of human galβ1,3galNAc α2,3-Sialyltransferase(hST3Gal II)
Author(s)
Y J Kim; K S Kim; S H Kim; C H Kim; Jung Hun Koh; In Seong Choe; S Tsuji; Y C Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 228, no. 2, pp. 324-327
Publication Year
1996
Abstract
A cDNA of human Galβ1,3GalNAc α2,3-sialyltransferase (hST3Gal II), which has been known to exhibit much more acceptor substrate preference for glycolipid than for O-linked oligosaccharides of glycoproteins, was isolated from the human liver cDNA library by plaque hybridization using the cDNA of mouse ST3Gal II (mST3Gal II) cloned previously as a probe. Comparative analysis of this cDNA with mST3Gal II indicates 89 and 94% homologies in the nucleotide and amino acid levels, respectively, between the two sequences in the predicted coding region. Northern analysis indicated that the expression of hST3Gal II mRNA is tissue-specific, it being prominent in skeletal muscle and heart, while that in lung and kidney is very low. This enzyme expressed in COS cells showed a similiar activity with that of mST3Gal II.
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/bbrc.1996.1660
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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