Molecular cloning and expression of human galβ1,3galNAc α2,3-Sialyltransferase(hST3Gal II)
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- Title
- Molecular cloning and expression of human galβ1,3galNAc α2,3-Sialyltransferase(hST3Gal II)
- Author(s)
- Y J Kim; K S Kim; S H Kim; C H Kim; Jung Hun Koh; In Seong Choe; S Tsuji; Y C Lee
- Bibliographic Citation
- Biochemical and Biophysical Research Communications, vol. 228, no. 2, pp. 324-327
- Publication Year
- 1996
- Abstract
- A cDNA of human Galβ1,3GalNAc α2,3-sialyltransferase (hST3Gal II), which has been known to exhibit much more acceptor substrate preference for glycolipid than for O-linked oligosaccharides of glycoproteins, was isolated from the human liver cDNA library by plaque hybridization using the cDNA of mouse ST3Gal II (mST3Gal II) cloned previously as a probe. Comparative analysis of this cDNA with mST3Gal II indicates 89 and 94% homologies in the nucleotide and amino acid levels, respectively, between the two sequences in the predicted coding region. Northern analysis indicated that the expression of hST3Gal II mRNA is tissue-specific, it being prominent in skeletal muscle and heart, while that in lung and kidney is very low. This enzyme expressed in COS cells showed a similiar activity with that of mST3Gal II.
- ISSN
- 0006-291X
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1006/bbrc.1996.1660
- Type
- Article
- Appears in Collections:
- Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
- Files in This Item:
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