Design and cloning of the gene for a novel insulin analogue,(B30-homoserine) human insulin

Cited 2 time in scopus
Metadata Downloads
Title
Design and cloning of the gene for a novel insulin analogue,(B30-homoserine) human insulin
Author(s)
D H Nam; Jung Hun Koh; S Y Lee
Bibliographic Citation
Archives of Pharmacal Research, vol. 16, no. 4, pp. 271-275
Publication Year
1993
Abstract
In order to prepare a novel human insulin analogue substituted with homoserine at B30 position, (B30-homoserine) human insulin, a synthetic gene was designed by linking directly a gene for B chain with that for A chain. This gene was constructed by enzymatic joining of 10 different synthetic oligonucleotides, and then inserted at the polylinker region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique was employed using amino terminal regions of lacZ gene up to ClaI or Hpal, and either of them has been located under tac promoter. The chemical induction of these fused genes by isopropyl-β-D-thiogalactopyranoside (IPTG) gave a satisfactory level of expression in Escherichia coli harboring the constructed plasmids. It was observed that the fused gene product as a single chain insulin precursor was produced more than 30% of total cell protein of E. coli as a form of inclusion body.
Keyword
recombinant human insulininsulin analogueinsulin [b30 homoserine]single chain insulin precursorgene fusion
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02977515
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.