Secretory expression of human αs₁-casein in Saccharomyces cerevisiae

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Title
Secretory expression of human αs₁-casein in Saccharomyces cerevisiae
Author(s)
Yoo Kyeong Kim; Dae Yeul Yu; Kang Hyun Ah; Sun Yoon; Bong Hyun Chung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 196-200
Publication Year
1999
Abstract
A recombinant human α(s1)-casein was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase, and human α(s1)- casein were used to direct the secretion of human α(s1)-casein into the extracellular medium. Among the three leader sequences tested, the native leader sequence of human α(s1)-casein was found to be the most efficient in the secretory expression of human α(s1)-casein, which implies that the native leader sequence of human α(s1)-casein might be used very efficiently for the secretory production of other heterologous proteins in yeast. The recombinant human α(s1)-casein was proteolytically cleaved as the culture proceeded. Therefore, an attempt was made to produce human α(s1)-casein using a S. cerevisiae mutant in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 72 h of culture, most of the human α(s1)-casein secreted by the wild type was cleaved, whereas more than 70% of the human α(s1)-casein secreted by yap3-disruptant remained intact. The results suggest that YAP3 might be involved in the internal cleavage of human α(s1)-casein expressed in yeast.
Keyword
Human α(s1)-caseinsecretionleader sequenceproteolysisSaccharomyces cerevisiae
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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