Vectors for rapid selection of intergrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1

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Title
Vectors for rapid selection of intergrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1
Author(s)
Michael O Agaphonov; Polina M Trushkina; Jung Hoon Sohn; Eui Sung Choi; Sang Ki Rhee; M D Ter-Avanesyan
Bibliographic Citation
Yeast, vol. 15, pp. 541-551
Publication Year
1999
Abstract
Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DLI. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in law (1-2), moderate (6-9) or high (up to 100) copy numbers. The vectors and mutants described are potentially useful for the construction of efficient producers of heterologous proteins in N. polymorpha.
Keyword
Hansenula polymorphaplasmidtransformationmultiple integration
ISSN
0749-503X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/(SICI)1097-0061(199905)15:7<541::AID-YEA392>3.0.CO;2-G
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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