Protective antitumor activity through dendritic cell immunization is mediated by NK cell as well as CTL activation

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Title
Protective antitumor activity through dendritic cell immunization is mediated by NK cell as well as CTL activation
Author(s)
Kwang Dong Kim; Jin Koo Kim; Se Jin Kim; In Seong Choe; Tai Wha Chung; Yong Kyung Choe; Jong-Seok Lim
Bibliographic Citation
Archives of Pharmacal Research, vol. 22, no. 4, pp. 340-347
Publication Year
1999
Abstract
Dendritic cells (DCs) are potent professional antigen-presenting cells (APC) capable of inducing the primary T cell response to antigen. Although tumor cells express target antigens, they are incapable of stimulating a tumor-specific immune response due to a defect in the costimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach using tumor-DC coculture to improve the antigen presenting capacity of tumor cells, which does not require a source of tumor-associated antigen. Immunization of a weakly immunogenic and progressive tumor cocultured with bone marrow-derived DCs generated an effective tumor vaccine. Immunization with the cocultured DCs was able to induce complete protective immunity against tumor challenges and was effective for the induction of tumor-specific CTL (cytotoxic T lymphocyte) activity. Furthermore, high NK cell activity was observed in mice in which tumors were rejected. In addition, immunization with tumor-pulsed DCs induced delayed tumor growth, but not tumor eradication in tumor-bearing mice. Our results demonstrate that coculture of DCs with tumors generated antitumor immunity due to the NK cell activation as well as tumor-specific T cell. This approach would be useful for designing tumor vaccines using DCs when the information about tumor antigens is limited.
Keyword
Dendritic cells (DCs)Colon tumorAntitumor immunity
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02979055
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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