Endoproteolytic processing of human parathyroid hormone in Saccharomyces cerevisiae mutants lacking genes encoding the Yap3, Mkc7, and Kex2 endoproteases

Cited 0 time in scopus
Metadata Downloads
Title
Endoproteolytic processing of human parathyroid hormone in Saccharomyces cerevisiae mutants lacking genes encoding the Yap3, Mkc7, and Kex2 endoproteases
Author(s)
Won A Choi; Hyun Ah Kang; Bong Hyun Chung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 679-682
Publication Year
1999
Abstract
When the human parathyroid hormone (hPTH) is expressed as a secretory product in S. cerevisiae, most of the secreted hPTH is internally cleaved by endoproteolytic processing. To investigate whether the yeast endoproteases such as Kex2p, Yap3p, and Mkc7p are involved in the endoproteolytic processing of hPTH in S. cerevisiae, hPTH was expressed in S. cerevisiae mutants deficient in one or two of the following well-known endoproteases such as Kex2p, Mkc7p, and Yap3p. Among these mutants, the yap3-disrupted (yap3Δ) and yap3/mkc7-disrupted (yap3Δmkc7Δ) yeasts showed a significant reduction in the extent of hPTH proteolysis. In contrast, the mkc7-disrupted (mkc7Δ) yeast did not reduce the proteolysis of hPTH as compared to the wild type. This suggests that Mkc7p is not involved in the endoproteolytic processing of hPTH. It was also found that the kex2-disrupted (kex2Δ) mutant was not able to secrete a detectable amount of hPTH.
Keyword
Human parathyroid hormonesecretory productionproteolysisendoproteasesS. cerevisiae
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.