DC Field | Value | Language |
---|---|---|
dc.contributor.author | Young Jun Kang | - |
dc.contributor.author | Young Shin Kim | - |
dc.contributor.author | Hyang Suk Hur | - |
dc.contributor.author | Hee Sun Kim | - |
dc.contributor.author | Hyo Jeong Hong | - |
dc.date.accessioned | 2017-04-19T08:56:26Z | - |
dc.date.available | 2017-04-19T08:56:26Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4850 | - |
dc.description.abstract | The complete (encoding 55 amino acids, aa) or partial (encoding aa 1-26) preS2 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathion-S-transferase (GST) gene and expressed under the control of the inducible tac promoter in Escherichia coli at 37°C. The fusion protein with the complete preS2 region was moderately expressed (8%) while the protein with the N-terminal 26 aa was expressed at a higher level, yielding about 20% of the total cellular proteins. The GST-preS2 (aa 1-26) protein, which contains the immunodominant epitope, was produced form the soluble protein fraction of the recombinant bacteria and purified by affinity chromatography using glutathione-agarose column. The purified preS2 fusion protein showed the antigenicity of preS2, as assessed by indirect and competitive ELISAs. | - |
dc.publisher | Springer | - |
dc.title | Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli | - |
dc.title.alternative | Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 380 | - |
dc.citation.startPage | 375 | - |
dc.citation.volume | 21 | - |
dc.contributor.affiliatedAuthor | Hyang Suk Hur | - |
dc.contributor.affiliatedAuthor | Hyo Jeong Hong | - |
dc.contributor.alternativeName | 강영준 | - |
dc.contributor.alternativeName | 김영신 | - |
dc.contributor.alternativeName | 허향숙 | - |
dc.contributor.alternativeName | 김희선 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 21, pp. 375-380 | - |
dc.identifier.doi | 10.1023/A:1005487105048 | - |
dc.subject.keyword | Escherichia coli | - |
dc.subject.keyword | Expression | - |
dc.subject.keyword | hepatitis B virus | - |
dc.subject.keyword | preS2 antigen | - |
dc.subject.local | Escherichia coli. | - |
dc.subject.local | escherichia coli | - |
dc.subject.local | Escherichia Coli | - |
dc.subject.local | Escherichia coli | - |
dc.subject.local | E.coli | - |
dc.subject.local | escherichia coil | - |
dc.subject.local | E. coli | - |
dc.subject.local | E. Coli | - |
dc.subject.local | expression | - |
dc.subject.local | Expression | - |
dc.subject.local | hepatitis B virus (HBV) | - |
dc.subject.local | Hepatitis B Virus | - |
dc.subject.local | Hepatitis B virus (HBV) | - |
dc.subject.local | hepatitis B virus | - |
dc.subject.local | hepatitis B Virus (HBV) | - |
dc.subject.local | Hepatitis B virus | - |
dc.subject.local | preS2 antigen | - |
dc.description.journalClass | Y | - |
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