Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli

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dc.contributor.authorYoung Jun Kang-
dc.contributor.authorYoung Shin Kim-
dc.contributor.authorHyang Suk Hur-
dc.contributor.authorHee Sun Kim-
dc.contributor.authorHyo Jeong Hong-
dc.date.accessioned2017-04-19T08:56:26Z-
dc.date.available2017-04-19T08:56:26Z-
dc.date.issued1999-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4850-
dc.description.abstractThe complete (encoding 55 amino acids, aa) or partial (encoding aa 1-26) preS2 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathion-S-transferase (GST) gene and expressed under the control of the inducible tac promoter in Escherichia coli at 37°C. The fusion protein with the complete preS2 region was moderately expressed (8%) while the protein with the N-terminal 26 aa was expressed at a higher level, yielding about 20% of the total cellular proteins. The GST-preS2 (aa 1-26) protein, which contains the immunodominant epitope, was produced form the soluble protein fraction of the recombinant bacteria and purified by affinity chromatography using glutathione-agarose column. The purified preS2 fusion protein showed the antigenicity of preS2, as assessed by indirect and competitive ELISAs.-
dc.publisherSpringer-
dc.titleEfficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli-
dc.title.alternativeEfficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number0-
dc.citation.endPage380-
dc.citation.startPage375-
dc.citation.volume21-
dc.contributor.affiliatedAuthorHyang Suk Hur-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.alternativeName강영준-
dc.contributor.alternativeName김영신-
dc.contributor.alternativeName허향숙-
dc.contributor.alternativeName김희선-
dc.contributor.alternativeName홍효정-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 21, pp. 375-380-
dc.identifier.doi10.1023/A:1005487105048-
dc.subject.keywordEscherichia coli-
dc.subject.keywordExpression-
dc.subject.keywordhepatitis B virus-
dc.subject.keywordpreS2 antigen-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localexpression-
dc.subject.localExpression-
dc.subject.localhepatitis B virus (HBV)-
dc.subject.localHepatitis B Virus-
dc.subject.localHepatitis B virus (HBV)-
dc.subject.localhepatitis B virus-
dc.subject.localhepatitis B Virus (HBV)-
dc.subject.localHepatitis B virus-
dc.subject.localpreS2 antigen-
dc.description.journalClassY-
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